Regulatory

Part:BBa_K3237027:Design

Designed by: Kristi Turton   Group: iGEM19_Lethbridge   (2019-10-16)


PcopM72 Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Design was based on the literature [1] but was used in a different way as to promote growth of microalgae with zinc present and cause cell death if accidentally released.


Source

The sequence was taken from the literature [1].

References

1.Čelešnik, H., Tanšek, A., Tahirović, A., Vižintin, A,. Mustar, J., Vidmar, V., and Dolinar, M. (2016) Biosafety of biotechnologically important microalgae: intrinsicsuicide switch implementation in cyanobacteriumSynechocystissp.PCC 6803. The Company of Biologists. 5, 519-528.